Bioedit An Important Software For Molecular Biology' title='Bioedit An Important Software For Molecular Biology' />Molecular cloning, characterization, and functional analysis of.Cc. BBMgene from camphor tree Cinnamomum camphora.L. Abstract. Baby boom BBM, as a transcription factor of AP2ERF family, has great potence in promoting somatic embryogenesis.In this study, a BBM gene from somatic embryos of camphor tree Cinnamomum camphora L., named Cc.BBM, was cloned and characterized.A partial fragment of 5.Cc. BBM was amplified by degenerate PCR based on the two conserved AP2 domains of other annotated BBMs.Full length c. DNA of Cc.BBM was obtained by a cloning strategy combing degenerate PCR and RACE technique.The open reading frame of Cc.BBM is 2. 16. 9 bp in length, which encodes 7.A comparison of the deduced amino acid sequence of Cc.BBM with BBM from other species showed high similarities, ranging from 5.The m. RNA levels of Cc.BBM in different tissues were detected by semi quantitative reverse transcription PCR RT PCR.Cc. BBM was expressed in most tissues, with highest expression level in somatic embryos, followed by roots, young fruits and flowers.Ectopic expression of Cc.BBM gene in Arabidopsis exhibited a variety of deformed and variegated leaves and short roots.No spontaneous somatic embryogenesis was observed in 3.S Cc. BBM transgenic plants.These results will help us understand the role of Cc.BBM in somatic embryogenesis in camphor tree.Molecular Biology Freeware for Windows.A. General below.B. Microarray next page.C. Java programs next page. 7 Wonders 2 Download Crackle on this page. A good places to start is Genamics SoftwareSeek.ORIGINAL PAPER Molecular characterization of Cryptosporidium in pigs in central Vietnam Sam Thi Nguyen Yasuhiro Fukuda Chika Tada Rintaro Sato Vu Vy Huynh.
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